ABSTRACT
@#This study was designed to investigate the therapeutic effect of anti-inflammatory tripeptide KdPT on ophthalmoxerosis. Male BALB/c mice, 8-week old, were treated with 0.2% benzalkonium chloride solution to establish the ophthalmoxerosis model. Four weeks after modeling, the mice were randomly divided into control group, positive group and the low, medium, high dose groups of KdPT. Each group was given normal saline, artificial tears and 1, 10, 100 μg/mL KdPT, respectively. After 3, 5, 7, 10 and 14 days of treatment, the morphology of the eye surface was observed, and the fluorescein sodium staining score was performed. The amount of tear secretion was measured by phenol red cotton thread and the right corneas were taken out for histopathological analysis after 14 days of treatment. Data showed that there was no significant abnormality in general state and the weight of mice in each group at each time point of treatment. After 14 days of treatment, KdPT can promote the secretion of tear, repair the damaged corneal epithelium, and showed a significant therapeutic effect on ophthalmoxerosis in mice. Based on the data, it is possible for KdPT to be developed as a novel drug for ophthalmoxerosis.
ABSTRACT
Introduction: Candida species are the major fungal pathogens of humans. Among them, Candida krusei have emerged as a notable pathogen with a spectrum of clinical manifestations and is known to develop resistance against azoles mainly fl uconazole. Anti-microbial peptides play important roles in the early mucosal defence against infection and are potent anti-fungal agents since they fi ght against fungal infection as well as have ability to regulate host immune defence system. The aim of the study was to synthesize a small anti fungal peptide. Materials and Methods: The series of tripeptides were synthesized and screened for antifungal activity against Candida strains according to CLSI guidelines. Toxicity effect of peptide was tested with human erythrocytes. The mode of action of peptide on fungus was resolved by scanning electron microscopy (SEM) studies Results: The tripeptide FAR showed a prominent anti fungal activity among the series. The minimum inhibitory concentration and minimum fungicidal concentration of tripeptide FAR was found to be 171.25 μg/ml and 685 μg/ml, respectively against Candida krusei. The therapeutic index was 2.9. The haemolytic experiment revealed that this peptide is non - toxic to human cells. The SEM studies showed disruption of cell wall and bleb-like surface changes and irregular cell surface. Conclusion: The peptide showed a signifi cant antifungal activity against C. krusei. Thus, it can set a platform for the design of new effective therapeutic agents against C. krusei.
ABSTRACT
Aim This study aimed to observe effects of tyroserleutide(tyrosyl-seryl-leucine,YSL) on the survival time of mice transplanted with the ascitic fluid-type hepatocarcinoma H_(22),as well as the T lymphocyte transformation and killing activity of NK cell impacted by YSL on mice bearing H_(22) tumor.Methods The model of ascetic fluid-type hepatocarcinoma H_(22) was established and the survival time of mice bearing H_(22) tumor treated by YSL was observed.MTT was used to observe the effect of lymphocyte transformation and killing activity of NK cells activated by YSL in vitro.Results YSL could significantly prolong the survival time of mice bearing ascetic fluid-type hepatocarcinoma H_(22).At doses of 5 and 50 ?g?kg~(1),YSL could advance the T lymphocyte transformation.At doses of 0.5,5 and 50 ?g?kg~(-1),YSL could enhance the killing activity of NK cells on mice bearing H_(22) tumor.Conclusion YSL can significantly prolong the survival time of mice bearing fluid-type hepatocarcinoma H_(22) and promote the effect of T lymphocyte transformation and NK cell killing activity.